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& Walbeehm, E

At the end of the experiments, the mice were sacrificed (cervical dislocation for short distribution time points and carbon dioxide asphyxia for longer ones), and the draining LNs (ipsilateral popliteal), the second LN in the chain (lumbar), and nondraining LNs (axillary and contralateral popliteal LNs) were rapidly dissected free (Figure 1A)

Fe + drives the Fenton reaction, producing unstable hydroxyl radicals that initiate lipid oxidation of PUFAs, forming conjugated dienes and eventually yielding toxic lipid peroxidation products such as 4-hydroxynonenal (4-HNE) and MDA